CD27 antibody (FITC)

Catalog No. ABIN396917

The Hamster Monoclonal anti-CD27 antibody has been validated for FACS, IHC, IHC (p) and IHC (fro). It is suitable to detect CD27 in samples from Human and Mouse.

Quick Overview for CD27 antibody (FITC) (ABIN396917)

Target: CD27 (CD27 Molecule (CD27))

Reactivity: Human, Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD27 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: LG-3A10

Product Details anti-CD27 Antibody (FITC)

Brand: IHC-plus™

Specificity: Anti-mouse CD27 Monoclonal Antibody is specific for the mouse CD27 receptor.

Purification: Protein G purified

Immunogen: MCD27 tag expressing clones AR0524 and AR0530.

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (0.5 μg/10 x10⁶), IHC, IHC-Fr, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide, EIA grade BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for CD27

Target: CD27 (CD27 Molecule (CD27))

Alternative Name: CD27

Background: Name/Gene ID: CD27
Family: TNF Receptor

Synonyms: CD27, CD27 antigen, CD27 Molecule, S152, T14, T-cell activation antigen CD27, CD27L receptor, T cell activation antigen S152, TNFRSF7, Tp55

Gene ID: 939

UniProt: P26842

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints