MCL-1 antibody

Catalog No. ABIN400763

The Mouse Monoclonal anti-MCL-1 antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect MCL-1 in samples from Human.

Quick Overview for MCL-1 antibody (ABIN400763)

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCL-1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 8C6D4B1

Product Details anti-MCL-1 Antibody

Specificity: This antibody recognizes Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: BCL2L3, Bcl-2-like protein 3, mcl1/EAT, EAT, r004, Induced myeloid leukemia celldifferentiation protein Mcl-1, Bcl-2-related protein

Purification: Protein G Chromatography.

Immunogen: Ni-NTA purified recombinant protein MCL1 expressed in E. Coli strain M15.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/10000. Western blot: 1/200-1/1000. Immunohistochemistry on Paraffin Sections: 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for MCL-1

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Alternative Name: MCL1

Background: MCL1 is an anti-apoptotic member of Bcl2 family originally isolated from the ML1 human myeloid leukemia cell line during phorbol ester-induced differentiation along the monocyte/macrophage pathway. Mcl1 localizes to the mitochondria, interacts with and antagonizes pro-apoptotic Bcl2 family members, and inhibits apoptosis by a number of cytotoxic stimuli. It is involved in programing of differentiation and concomitant maintenance of viability but not of proliferation. Isoform 1 inhibits apoptosis while isoform 2 promotes it. Expression increases early during phorbol-ester induced differentiation along the monocyte/macrophage pathway in myeloid leukemia cell lines ML1.Synonyms: BCL2L3, Bcl-2-like protein 3, Bcl-2-related protein, EAT, Induced myeloid leukemia cell differentiation protein Mcl-1, mcl1/EAT, r004

Gene ID: 4170

UniProt: Q07820

Pathways: MAPK Signaling