Glutathione Peroxidase 1 antibody

Catalog No. ABIN400775

The Mouse Monoclonal anti-Glutathione Peroxidase 1 antibody has been validated for WB, IP, IHC (p) and EIA. It is suitable to detect Glutathione Peroxidase 1 in samples from Human and Rat.

Quick Overview for Glutathione Peroxidase 1 antibody (ABIN400775)

Target: Glutathione Peroxidase 1 (GPX1)

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Glutathione Peroxidase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 13B2AF

Product Details anti-Glutathione Peroxidase 1 Antibody

Specificity: Recognizes Glutathione Peroxidase (GPX1).

Cross-Reactivity (Details): Species reactivity (expected):Human and Rat.
Species reactivity (tested):Mouse.

Characteristics: Synonyms: GSHPx-1, Cellular glutathione peroxidase

Purification: Purified

Immunogen: Recombinant Mouse protein purified from E. coli (Gpx1).

Isotype: IgG1

Application Details

Application Notes: ELISA. Western Blot: 1/5000. Immunoprecipitation. Immunohistochemistry on Paraffin Sections: 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: HEPES containing 0.15 M Sodium Chloride, 0.01 % BSA as stabilizer, 0.03 % Sodium Azide as preservative and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Glutathione Peroxidase 1

Target: Glutathione Peroxidase 1 (GPX1)

Alternative Name: Glutathione Peroxidase 1 / GPX1

Background: Glutathione peroxidases(GPxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. This protein is one of the most important antioxidant enzymes in humans. This protein has been reported to protect cultured breast cancer cells from CD95-induced apoptosis and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell growth and increases resistance to toxic challenges. There are several isoforms which differ in their primary structure and localization. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis specific secretory GPx(eGPxor GPx5)isoforms, possess selenocysteine at the active site. One allele of the Glutathione Peroxidase 1 gene is significantly associated with an increased breast cancer risk.Synonyms: Cellular glutathione peroxidase, GSHPx-1

Gene ID: 2876

UniProt: P07203

Pathways: Thyroid Hormone Synthesis, Sensory Perception of Sound, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome