CD34 antibody

Catalog No. ABIN400780

This Rat Monoclonal antibody specifically detects CD34 in FACS, IP and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for CD34 antibody (ABIN400780)

Target: CD34

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MEC 14-7

Product Details anti-CD34 Antibody

Specificity: Recognizes the Mouse CD34 cell surface glycoprotein. Mouse CD34 is expressed by hematopoietic progenitor cells in the bone marrow and also by endothelial cells.

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Characteristics: Synonyms: Hematopoietic progenitor cell marker, Hematopoietic progenitor cell antigen CD34

Purification: Protein G Chromatography.

Isotype: IgG2a

Application Details

Application Notes: Immunoprecipitation: 2-10 μg/mL. Flow Cytometry: Use 10 μL of 1/25-1/50 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Paraffin Sections: 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one monthor (Add 40-50% Sterile Glycerol) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD34

Target: CD34

Alternative Name: CD34

Background: The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.Synonyms: Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker

Gene ID: 12490

UniProt: Q64314