RNH1 antibody (AA 7-461)

Catalog No. ABIN400852

This Mouse Monoclonal antibody specifically detects RNH1 in WB, IHC (p) and EIA. It exhibits reactivity toward Human and Mouse.

Quick Overview for RNH1 antibody (AA 7-461) (ABIN400852)

Target: RNH1 (Ribonuclease/angiogenin Inhibitor 1 (RNH1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This RNH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: AT1H23

Product Details anti-RNH1 Antibody

Binding Specificity: AA 7-461

Specificity: The antibody recognizes Human and Mouse RNH1 (7-461). Other species not tested.

Characteristics: Synonyms: Ribonuclease/angiogenin inhibitor 1, RAI, RNH, PRI, Placental ribonuclease inhibitor

Purification: Protein-G affinity chromatography

Immunogen: Recombinant Ribonuclease inhibitor 1 (RNH1) (7-461 aa) purified from E. coli

Isotype: IgG2a

Application Details

Application Notes: ELISA. Western blot (1/1,000-1/2,000). Immunohistochemistry on Paraffin Sections (5 μg/mL). This RNH1 antibody was validatedfor use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE)human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubationwith the primary antibody, slides were incubated with biotinylated secondary antibody,followed by alkaline phosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for up to two weeks or (in aliquots) at -20 °C forlonger. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for RNH1

Target: RNH1 (Ribonuclease/angiogenin Inhibitor 1 (RNH1))

Alternative Name: Ribonuclease Inhibitor (RNH1)

Background: Ribonuclease inhibitor 1 (RNH1) the placental ribonuclease inhibitor, is an acid protein with a molecular weight of 50 kDa. RNH1 is a member of a family of proteinaceous cytoplasmic RNase inhibitors that are expressed in many tissues and bind to both intracellular and extracellular RNases in the cytosol. RNH1 is a role in abolishing the ribonucleolytic and angiogenic activities of angiogenin.Synonyms: PRI, Placental ribonuclease inhibitor, RAI, RNH, Ribonuclease/angiogenin inhibitor 1

Gene ID: 6050

UniProt: P13489