PDPK1 antibody (AA 29-436)

Catalog No. ABIN400866

The Mouse Monoclonal anti-PDPK1 antibody (Clone AT2D6) (ABIN400866) specifically detects PDPK1 in WB and EIA. The antibody is reactive with Human and Rat samples.

Quick Overview for PDPK1 antibody (AA 29-436) (ABIN400866)

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This PDPK1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: AT2D6

Product Details anti-PDPK1 Antibody

Binding Specificity: AA 29-436

Specificity: The antibody recognizes human and rat PDK1. Other species not tested.

Characteristics: Synonyms: Pyruvate dehydrogenase kinase isoform 1, Pyruvate dehydrogenase [lipoamide] kinaseisozyme 1 mitochondrial

Purification: Protein-G affinity chromatography

Immunogen: Recombinant human PDK1 (29-436 aa) purified from E. coli

Isotype: IgG1

Application Details

Application Notes: ELISA. Western blot (1: 250 - 1: 1,000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for up to two weeks or (in aliquots) at -20 °C or -70 °Cfor longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for PDPK1

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Alternative Name: PDK1

Background: PDK1 (pyruvate dehydrogenase kinase isoform 1) is involved in the regulation of enzymatic activity of mammalian pyruvate dehydrogenase (PDH) that is a part of a mitochondrial multienzyme complex to catalyze the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. PDK1 has been found to serve as an effective therapeutic target for inhibition of glioblastoma growth.Synonyms: Pyruvate dehydrogenase [lipoamide] kinase isozyme 1 mitochondrial, Pyruvate dehydrogenase kinase isoform 1

Gene ID: 5163

UniProt: Q15118

Pathways: PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals