CENPQ antibody

Catalog No. ABIN401060

The Rabbit Polyclonal anti-CENPQ antibody is suitable to detect CENPQ in samples from Human, Mouse, Rat, Cow, Dog, Horse, Macaque and Pig. It has been validated for WB, IF and EIA.

Quick Overview for CENPQ antibody (ABIN401060)

Target: CENPQ (Centromere Protein Q (CENPQ))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Macaque, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CENPQ antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-CENPQ Antibody

Specificity: This antibody is specific for CENP-Q protein.

Cross-Reactivity (Details): Species reactivity (expected):Macaque, Horse, Bovine, Canine, Porcine, Mouse, Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography on protein A

Immunogen: Prepared from whole rabbit serum produced by repeated immunizations with full-length human CENP-Q recombinant protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1: 20,000 - 1: 30,000. Immunofluorescence. Western Blot: 1: 2,000 - 1: 2,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.15 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for CENPQ

Target: CENPQ (Centromere Protein Q (CENPQ))

Alternative Name: CENP-Q

Background: Cenp-Q (also known as centromere protein Q or CENPQ) is a nuclear/centromeric protein that is one of the critical components that constitutes the CENP-O complex at the kinetochores and appears to stabilize PBIP1/CENP-U(50)/MLF1IP in the complex. This complex is important for proper recruitment of polo-like kinase 1 (Plk1) to the mitotic kinetochores. A failure in this process results in improper microtubule attachment to the kinetochores and chromosome missegregation that ultimately lead to aneuploidy.Synonyms: C6orf139, CENPQ, Centromere protein Q, chromosome 6 open reading frame 139

Gene ID: 55166

NCBI Accession: NP_060602

UniProt: Q7L2Z9