ATPase (Na/K) antibody

Catalog No. ABIN401097

The Chicken Polyclonal anti-ATPase (Na/K) antibody (ABIN401097) specifically detects ATPase (Na/K) in WB, IHC (fro) and EIA. The antibody is reactive with Dog samples.

Quick Overview for ATPase (Na/K) antibody (ABIN401097)

Target: ATPase (Na/K)

Reactivity: Dog

Host: Chicken

Clonality: Polyclonal

Conjugate: This ATPase (Na/K) antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Product Details anti-ATPase (Na/K) Antibody

Specificity: This antibody reacts to Sodium Potassium ATPase.

Purification: Ion exchange chromatography

Immunogen: ATPase, Sodium, Potassium [Canine Kidney]

Isotype: Ig Fraction

Application Details

Application Notes: Western Blot: 1/100 to 1/500. ELISA: 1/1,000 to 1/6,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.1 mL of deionized water (or equivalent).

Concentration: 10.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for ATPase (Na/K)

Target: ATPase (Na/K)

Background: The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP dependent transport of sodium and potassium across the cell membrane. This membrane bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium / potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families. The different isoforms of the sodium / potassium ATPase exhibit tissue specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell specific expression. The alpha subunit has been found in kidney, brain, heart, and to a lesser extent liver, skeletal and smooth muscle.Synonyms: ATPase Sodium/Potasium, Sodium pump 1, Sodium/potassium dependent ATPase