GM-CSF antibody

Catalog No. ABIN401109

The Rabbit Polyclonal anti-GM-CSF antibody (ABIN401109) specifically detects GM-CSF in WB, Neut and EIA. The antibody is reactive with Mouse samples.

Quick Overview for GM-CSF antibody (ABIN401109)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GM-CSF antibody is un-conjugated

Application: Western Blotting (WB), Neutralization (Neut), Enzyme Immunoassay (EIA)

Product Details anti-GM-CSF Antibody

Specificity: This antibody reacts to Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF).

Purification: Purified

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse GM-CSF protein.

Isotype: IgG

Application Details

Application Notes: ELISA: 1/20000 - 1/100000. Western Blot: 1/500 - 1/2000. Neutralization: 1/400.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.1 mL of deionized water (or equivalent).

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: GM-CSF

Background: Granulocyte Macrophage Colony Stimulating Factor (also known as GM-CSF, Colony-stimulating factor, CSF, sargramostim and molgramostin) is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors show significant homologies with other receptors for hematopoietic growth factors, including IL2-beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors.Synonyms: CSF2, Colony stimulating factor 2, GMCSF, Granulocyte-macrophage colony-stimulating factor, Molgramostin, Sargramostim

Gene ID: 12981

NCBI Accession: NP_034099

UniProt: P01587

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin