KLH antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN401179

This Rabbit Polyclonal antibody specifically detects KLH in WB, IHC (fro), EIA and DB. It exhibits reactivity toward Keyhole Limpet.

Quick Overview for KLH antibody (Alkaline Phosphatase (AP)) (ABIN401179)

Target: KLH (Keyhole Limpet Hemocyanin (KLH))

Reactivity: Keyhole Limpet

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLH antibody is conjugated to Alkaline Phosphatase (AP)

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Dot Blot (DB)

Product Details anti-KLH Antibody (Alkaline Phosphatase (AP))

Purification: Affinity chromatography

Immunogen: KLH (Keyhole Limpet Hemocyanin)

Isotype: IgG

Application Details

Application Notes: Western BLot. ELISA: 1/3,000 - 1/13,500. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.005 M Magnesium Chloride, 0.0005 M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0 containing 10 mg/mL Bovine Serum Albumin (BSA) and 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for KLH

Target: KLH (Keyhole Limpet Hemocyanin (KLH))

Background: KLH (Keyhole Limpet Hemocyanin) is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50 kDa subunits.Synonyms: Keyhole Limpet Hemocyanine