PRKDC antibody (Thr2609)

Catalog No. ABIN401263

This Rabbit Polyclonal antibody specifically detects PRKDC in WB and EIA. It exhibits reactivity toward Human, Mouse, Rat, Chicken, Dog, Horse and Chimpanzee.

Quick Overview for PRKDC antibody (Thr2609) (ABIN401263)

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Reactivity: Human, Mouse, Rat, Chicken, Dog, Horse, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKDC antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PRKDC Antibody

Binding Specificity: Thr2609

Specificity: This antibody reacts with phosphorylated pThr2609 of DNA PKcs. Reactivity with non-phosphorylated Human DNA PKcs is minimal. Cross-reactivity with DNA PKcs homologues from other sources has not been determined.

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee (100 %), Mouse, Rat, Dog, Chicken.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.

Immunogen: Synthetic peptide corresponding to amino acids surrounding Thr 2609 of Human DNA PKcs

Isotype: IgG

Application Details

Application Notes: ELISA: 1/300,000. Western blot: 1/500-1/2,000. Expect a band approx. 460 kDa in size corresponding to DNA PKcsin the appropriate cell lysate or extract. Best western blotting results are seen when Immunoprecipitation is performed prior todetection. This antibody detects an inducible signal at the correct height after DNAdamage. However, the antibody may also detect an IR-inducible signal in cells lacking DNAPKcs (MO59J cells) at the same size as DNA PKcs if western blotting is performed directly. We believe that this additional band is 53BP1 that runs at the same size as DNA PKcs and isalso phosphorylated in an IR-dependent manner. Similar results were seen with anotherphospho DNA PKcs antibody indicating that this result is general to antibodies to thisphospho site, rather than specific to this antibody.

Restrictions: For Research Use only

Handling

Concentration: 0.93 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PRKDC

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Alternative Name: DNA-PKcs / PRKDC / XRCC7

Background: DNA dependent Protein Kinase (also called DNAPK, DNPK1, HYRC1, Protein Kinase DNA Activated Catalytic Polypeptide, XRCC7 and p460) consists of the 460 kDa DNA PKcs and a heterodimeric regulatory complex comprised of p70 Ku and p80 Ku (Ku autoantigen). DNA PKcs is a nuclear protein serine/threonine kinase present in a wide variety of eukaryotic species. DNA PKcs phosphorylates transcription factors, Sp1, Oct-1, p53 and SV40 large T antigen. DNA PKcs is involved in repairing double stranded DNA breaks. At the onset of apoptosis, DNA PKcs is rapidly inactivated by cleavage of the catalytic subunit into smaller polypeptides. Proteolysis of DNA PKcs is inhibited by the cysteine protease inhibitors iodoacetamide and N-ethylmaleimide. Alternative splicing can occur for this protein to produce at least two isoforms.Synonyms: DNA-dependent protein kinase catalytic subunit, DNPK1, HYRC, HYRC1, p460

Molecular Weight: 469.1 kDa

Gene ID: 5591

NCBI Accession: NP_001075109

UniProt: P78527

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response