FANCF antibody (Internal Region)

Catalog No. ABIN401270

The Rabbit Polyclonal anti-FANCF antibody is suitable to detect FANCF in samples from Human and Chimpanzee. It has been validated for WB and EIA.

Quick Overview for FANCF antibody (Internal Region) (ABIN401270)

Target: FANCF (Fanconi Anemia, Complementation Group F (FANCF))

Reactivity: Human, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FANCF antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-FANCF Antibody

Binding Specificity: Internal Region

Specificity: This affinity purified antibody is directed against FANCF protein.

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee (100 %).
Species reactivity (tested):Human.

Purification: Immunoaffinity chromatography

Immunogen: Synthetic peptide corresponding to an internal amino acid sequence of human FANCF

Isotype: IgG

Application Details

Application Notes: ELISA: 1: 15,000 - 1: 60,000. Western blot: 1: 1,000 - 1: 5,000 (Expect a band approximately 42 kDa in size correspondingto FANCF in the appropriate human tissue).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.19 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for FANCF

Target: FANCF (Fanconi Anemia, Complementation Group F (FANCF))

Alternative Name: FANCF

Background: FANCF (also called Protein FACF or Fanconi Anemia Group F protein) is involved in DNA repair, perhaps specifically with post-replication repair or a cell cycle checkpoint function. FANCF has also been implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. FANCF belongs to the multi-subunit Fanconi Anemia (FA) complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. The complex is not found in FA patients. FANCF is found within the nucleus. Defects in FANCF are a cause of Fanconi anemia (FA). FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopaenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level, it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.Synonyms: FACF, Fanconi anemia group F protein

Gene ID: 2188

NCBI Accession: NP_073562

UniProt: Q9NPI8

Pathways: DNA Damage Repair