ELISA: 1: 10,000 - 1: 40,000. Western blot: 1: 500 - 1: 2,000, detects a band of approximately 96.0 kDa (predictedmolecular weight: 72.2 kDa). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.10 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
Target
WRNIP1
(Werner Helicase Interacting Protein 1 (WRNIP1))
Werner's syndrome is a rare autosomal recessive disorder characterized by premature aging. Werner helicase interacting protein 1 (WHIP) interacts with the N-terminal portion of Werner protein, which contains an exonuclease domain. This protein shows homology to replication factor C family proteins, and is conserved from E. coli to human. Studies in yeast suggest that this gene product may influence the aging process. A second isoform exists (WHIP2).Synonyms: WHIP, WRNIP1, Werner helicase-interacting protein 1