UBASH3B antibody (C-Term)

Catalog No. ABIN401302

This Rabbit Polyclonal antibody specifically detects UBASH3B in WB and EIA. It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for UBASH3B antibody (C-Term) (ABIN401302)

Target: UBASH3B (STS1) (Cbl-Interacting Protein Sts-1 (STS1))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBASH3B antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-UBASH3B Antibody

Binding Specificity: C-Term

Specificity: This antibody is directed against Sts-1 protein.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Human.
Species reactivity (tested):Mouse.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to the C-terminus of mouse Sts-1

Isotype: IgG

Application Details

Application Notes: ELISA: 1/2,000 - 1/10,000. Western Blot: 1/500 - 1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.63 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for UBASH3B

Target: UBASH3B (STS1) (Cbl-Interacting Protein Sts-1 (STS1))

Alternative Name: UBASH3B / STS1

Background: Sts-1 is a protein that inhibits endocytosis of epidermal growth factor receptor (EGFR) and platelet-derived growth factor receptor. Sts-1 and Sts-2 (formerly p70 and Clip4, respectively) have been found to interact with Cbl, an ubiquitin ligase that plays a critical role in attenuation of receptor tyrosine kinase signaling by inducing ubiquitination and promoting their sorting for endosomal degradation. Sts-1 and Sts-2 contain SH3 domains that interact with Cbl, Ub-associated domains, which bind directly to mono-Ub or to the EGFR/Ub chimera, as well as phosphoglycerate mutase domains that mediate oligomerization of Sts-1/2. Sts-1 and Sts-2 also have been found to negatively regulate signaling pathways that control T cell receptors, which in turn affect the extent and duration of the T cell response to foreign pathogens.Synonyms: Cbl-interacting protein p70, KIAA1959, STS-1, Suppressor of T-cell receptor signaling 1, Ubiquitin-associated and SH3 domain-containing protein B

Gene ID: 84959

NCBI Accession: NP_116262

UniProt: Q8TF42