HDAC1 antibody (AA 466-482)

Catalog No. ABIN401309

The Rabbit Polyclonal anti-HDAC1 antibody is suitable to detect HDAC1 in samples from Human, Mouse, Rat, Cow, Chicken and Chimpanzee. It has been validated for WB, IHC (p) and EIA.

Quick Overview for HDAC1 antibody (AA 466-482) (ABIN401309)

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Reactivity: Human, Mouse, Rat, Cow, Chicken, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-HDAC1 Antibody

Binding Specificity: AA 466-482

Specificity: This antibody is directed against HDAC-1 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Chimpanzee, Bovine, Chicken.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids 466-482 of Human HDAC-1

Isotype: IgG

Application Details

Application Notes: Western Blot: 1/1,000 - 1/5,000. ELISA: 1/10,000 - 1/50,000. Immunohistochemistry: 1/200 - 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.33 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for HDAC1

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Alternative Name: HDAC1

Background: HDAC1 (also known as HD1, histone deacetylase 1, RPD3, RPD3L1) belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.Synonyms: HD1, Histone deacetylase 1, RPD3L1

Gene ID: 3065

NCBI Accession: NP_004955

UniProt: Q13547

Pathways: Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Mitotic G1-G1/S Phases, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Negative Regulation of intrinsic apoptotic Signaling, Embryonic Body Morphogenesis