CTCFL antibody (AA 9-26)

Catalog No. ABIN401325

The Rabbit Polyclonal anti-CTCFL antibody (ABIN401325) specifically detects CTCFL in WB and EIA. The antibody is reactive with Human and Chimpanzee samples.

Quick Overview for CTCFL antibody (AA 9-26) (ABIN401325)

Target: CTCFL (CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL))

Reactivity: Human, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTCFL antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CTCFL Antibody

Binding Specificity: AA 9-26

Specificity: This antibody is directed against the BORIS protein.

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding aa 9-26 of human BORIS protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/2,000 - 1/10,000. Western Blot: 1/200 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.28 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for CTCFL

Target: CTCFL (CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL))

Alternative Name: CTCFL / BORIS

Background: BORIS (Brother of the Regulator of Imprinted Sites) also known as CCCTC-binding factor-like protein, is normally only expressed in the testis and expressed in a mutually exclusive manner with CTCF during male germ cell development. However, previous studies have shown that BORIS is abnormally activated in a wide range of human cancers. Expression of BORIS in normally BORIS-negative cells promotes cell growth that may lead to transformation. BORIS maps to the cancer-associated amplification region thought to contain an oncogene or dominant-immortalizing gene. BORIS is a candidate protein for the epigenetic reprogramming factor acting in the male germ line. BORIS is found in both the nucleus and cytoplasm.Synonyms: Brother of the regulator of imprinted sites, CCCTC-binding factor, CT27, CTCF paralog, CTCF-T, CTCF-like protein, Cancer/testis antigen 27, Transcriptional repressor CTCFL, Zinc finger protein CTCF-T

Gene ID: 140690

NCBI Accession: NP_542185

UniProt: Q8NI51