CRTC1 antibody (AA 19-34)

Catalog No. ABIN401346

The Rabbit Polyclonal anti-CRTC1 antibody (ABIN401346) specifically detects CRTC1 in WB and EIA. The antibody is reactive with Human, Mouse, Rat, Cow and Zebrafish (Danio rerio) samples.

Quick Overview for CRTC1 antibody (AA 19-34) (ABIN401346)

Target: CRTC1 (CREB Regulated Transcription Coactivator 1 (CRTC1))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRTC1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CRTC1 Antibody

Binding Specificity: AA 19-34

Specificity: This antibody is directed against MECT1 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Zebrafish, Pufferfish, Bovine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids 19-34 of human MECT1 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/10,000 - 1/40,000. Western Blot: 1/500 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.95 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for CRTC1

Target: CRTC1 (CREB Regulated Transcription Coactivator 1 (CRTC1))

Alternative Name: CRTC1 / MECT1

Background: MECT1 (also known as MucoEpidermoid Carcinoma Translocated 1, Transducer of regulated cAMP response elementbinding protein 1, TORC1, and Transducer of CREB protein 1) is a nuclear protein that functions as a transcriptional coactivator for CREB1, which activates transcription through both consensus and variant cAMP response element (CRE) sites. MECT1does not appear to modulate CREB1 DNA-binding activity but enhances the interaction of CREB1 with TAF4/TAFII-130. MECT1 translocates with MAML2 (MasterMind-Like Protein 2) to yield a fusion oncogene: t(11,19) (q21,p13). This translocation occurs in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas. The novel fusion product that results disrupts the Notch signaling pathway. The fusion protein consists of the N-terminus of MECT1 joined to the C-terminus of MAML2. The reciprocal fusion protein consisting of the N-terminus of MAML2 joined to the C-terminus of MECT1 has been detected in a small number of mucoepidermoid carcinomas. Multiple isoforms have been reported for the MECT1 protein.Synonyms: CREB-regulated transcription coactivator 1, KIAA0616, Mucoepidermoid carcinoma translocated protein 1, TORC1, Transducer of regulated cAMP response element-binding protein 1, WAMTP1

Gene ID: 23373

NCBI Accession: NP_001091952

UniProt: Q6UUV9