EIF3E antibody (C-Term)

Catalog No. ABIN401422

This Rabbit Polyclonal antibody specifically detects EIF3E in WB and EIA. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog and Monkey.

Quick Overview for EIF3E antibody (C-Term) (ABIN401422)

Target: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Reactivity: Human, Mouse, Rat, Cow, Dog, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3E antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-EIF3E Antibody

Binding Specificity: C-Term

Specificity: This antibody is specific for Mouse eIF3S6/Int6 protein.

Cross-Reactivity (Details): Species reactivity (expected):Human, Rat, Canine, Bovine, Monkey.
Species reactivity (tested):Mouse.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to a region near the C-terminus of mouse EIF3S6/Int6 . Genename: EIF3E

Isotype: IgG

Application Details

Application Notes: Suitable for use in ELISA (1/80,000) and Western Blot (1/1,000). Expect a band approx. 48 kDa in size corresponding to eIF3S6/Int6 by Western blotting in theappropriate cell lysate or extract. This antibody is capable of detecting bothover-expressed and endogenous eIF3S6/Int6.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.8 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for EIF3E

Target: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Alternative Name: EIF3E / EIF3S6

Background: Int6 is a candidate tumor suppressor in multiple neoplasms, and in particular, breast and lung cancers. The Int6 locus was initially identified as a common insertion site (CIS) in a genetic screen for transforming sequences in a breast cancer mouse model system. Insertion of mouse mammary tumor virus (MMTV) into this locus results in the production of an amino-terminal truncated gene product. Expression of the truncated Int6 product corresponds to cellular transformation in both in vivo and in vitro systems. This gene product plays a role in regulating translation initiation and is a component of the eIF3 translation initiation complex. There is evidence that suggests that Int6 may impart a negative role in the general translational machinery while promoting an increase in the expression of a subset of stress-responsive genes. Taken together, it is of great interest to further study the mechanism by which Int6 is involved in regulating cell growth.Synonyms: Eukaryotic translation initiation factor 3 subunit 6, Eukaryotic translation initiation factor 3 subunit E, INT6, Viral integration site protein INT-6 homolog, eIF-3 p48

Gene ID: 16341

NCBI Accession: NP_032414

UniProt: P60229

Pathways: Ribonucleoprotein Complex Subunit Organization, Hepatitis C