MYB antibody (Internal Region)

Catalog No. ABIN401455

The Rabbit Polyclonal anti-MYB antibody is suitable to detect MYB in samples from Human, Mouse, Rat, Horse, Pig, Monkey, Bonobo, Chimpanzee, Gorilla, Opossum and Orang-Utan. It has been validated for WB and EIA.

Quick Overview for MYB antibody (Internal Region) (ABIN401455)

Target: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Reactivity: Human, Mouse, Rat, Horse, Pig, Monkey, Bonobo, Chimpanzee, Gorilla, Opossum, Orang-Utan

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYB antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-MYB Antibody

Binding Specificity: Internal Region

Specificity: This antibody reacts with endogenous c-Myb protein and also SUMOylated overexpressed c-Myb protein in transfected Cos7 cells.

Purification: Affinity chromatography

Immunogen: Peptide corresponding to an internal portion of the c-Myb protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/10,000 - 1/25,000. Western Blot: 1/250 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for MYB

Target: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Alternative Name: MYB

Background: The transcription factor c-Myb plays an important role in the development of all hematopoietic lineages during definitive hematopoiesis. Its critical role in hematopoiesis has been first demonstrated in experiments with targeted disruption of the c-Myb gene where deletion of both alleles of the c-Myb gene was embryonally lethal due to severe anemia. c-Myb is a short-lived sequence specific DNA-binding protein that regulates transcription of several important genes involved directly in cellular processes such as proliferation, differentiation, and apoptosis. Posttranslational modifications such as phosphorylation, acetylation, ubiquitination, and conjugation of SUMO proteins are crucial for modulation of the transactivation activity of c-Myb.Synonyms: Proto-oncogene c-Myb, Transcriptional activator Myb, c-Myb

Gene ID: 17863

NCBI Accession: NP_001185843

UniProt: P06876

Pathways: Chromatin Binding, Positive Regulation of Immune Effector Process