Tyrosine Hydroxylase antibody (pSer19)

Catalog No. ABIN401584

This Rabbit Polyclonal antibody specifically detects Tyrosine Hydroxylase in IF. It exhibits reactivity toward Rat.

Quick Overview for Tyrosine Hydroxylase antibody (pSer19) (ABIN401584)

Target: Tyrosine Hydroxylase (TH)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tyrosine Hydroxylase antibody is un-conjugated

Application: Immunofluorescence (IF)

Product Details anti-Tyrosine Hydroxylase Antibody

Binding Specificity: pSer19

Specificity: Antibody AP09482PU detects endogenous levels of Tyrosine Hydroxylase only when phosphorylated at Serine 19.

Purification: Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Tyrosine Hydroxylase around the phosphorylation site of Serine19 (A-V-Sp-E-Q).

Application Details

Application Notes: Immunofluorescence: 1/100approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at-20 °C.

Target Details for Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Alternative Name: Tyrosine 3-Monooxygenase (TH)

Background: Tyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson's diseaseSynonyms: TYH, Tyrosine 3-hydroxylase

Gene ID: 7054

NCBI Accession: NP_000351

UniProt: P07101

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour