IKBKB antibody (Tyr199)

Catalog No. ABIN401637

The Rabbit Polyclonal anti-IKBKB antibody has been validated for IF. It is suitable to detect IKBKB in samples from Human, Mouse and Rat.

Quick Overview for IKBKB antibody (Tyr199) (ABIN401637)

Target: IKBKB (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IKBKB antibody is un-conjugated

Application: Immunofluorescence (IF)

Product Details anti-IKBKB Antibody

Binding Specificity: Tyr199

Specificity: IKK-β Antibody detects endogenous levels of total IKK-β protein.

Purification: Affinity chromatography

Immunogen: Synthesized non-phosphopeptide derived from human IKK-beta around the phosphorylation site of tyrosine 199 (Q-K-YP-T-V)

Application Details

Application Notes: Immunofluorescence: 1/100 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for IKBKB

Target: IKBKB (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))

Alternative Name: IKBKB / IKKB

Background: IKK beta (I-Kappa-B kinase-beta) is a member of the IKK complex which is composed of IKK alpha, IKK beta, IKK gamma and IKAP. Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. IKK beta has been shown to activate NF-kB and phosphorylate IKB alpha and beta. Phosphorylation of 2 sites at the activation loop of IKK beta is essential for activation of IKK by TNF and IL1. Once activated, IKK beta autophosphorylates which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKK beta activity can also be regulated by MEKK1.Synonyms: I-kappa-B kinase 2, I-kappa-B-kinase beta, IKK-B, IKK-beta, IKK2, Inhibitor of nuclear factor kappa-B kinase subunit beta, NFKBIKB, Nuclear factor NF-kappa-B inhibitor kinase beta

Gene ID: 3551

NCBI Accession: NP_001547

UniProt: O14920

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins