Myc Tag antibody (Biotin)

Catalog No. ABIN401744

Product Details anti-Myc Tag Antibody (Biotin)

Target: Myc Tag

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Myc Tag antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Sequence: EQKLISEEDL

Specificity: This antibody is directed against human c-myc.

Characteristics: Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule

Purification: Affinity chromatography

Immunogen: Purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.

Application Details

Application Notes: ELISA: 1/4,000 - 1/20,000. Western Blot: 1/400 - 1/2,000. Immunohistochemistry: 1/400 - 1/2,000.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.1 mL of deionized water (or equivalent). For extended storage mix with glycerol to 50 % .

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA as stabilizer and 0.01 % (w/v) Sodium Azide as perservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store undiluted at 2-8 °C. DO NOT FREEZE!

Target Details for Myc Tag

Target: Myc Tag

Alternative Name: C-Myc Epitope Tag (Myc Tag Products)

Target Type: Tag

Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.Synonyms: c-myc tag, myc tag, myc-tag

Gene ID: 4609

NCBI Accession: NP_002458

UniProt: P01106