Albumin antibody (Biotin)

Catalog No. ABIN401768

The Rabbit Polyclonal anti-Albumin antibody is suitable to detect Albumin in samples from Human. It has been validated for WB, EIA and IHC (fro).

Quick Overview for Albumin antibody (Biotin) (ABIN401768)

Target: Albumin (ALB)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Albumin antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Albumin Antibody (Biotin)

Specificity: This antibody reacts to Serum Albumin.

Characteristics: Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule

Purification: Affinity chromatography on Protein A

Immunogen: Human Serum Albumin

Application Details

Application Notes: Western Blot: 1/1,000 - 1/5,000. ELISA: 1/170,000. Immunohistochemistry: 1/500 - 1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL of deionized water (or equivalent). For extended storage add glycerol to 50 %

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL Bovine Serum Albumin (BSA) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at -20 °C. After reconstitution, store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Albumin

Target: Albumin (ALB)

Alternative Name: Albumin

Background: Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.Synonyms: ALB, BSA, HSA, Serum Albumin

Gene ID: 213

NCBI Accession: NP_000468

UniProt: P02768

Pathways: Lipid Metabolism