Recombinant RFP antibody (AZDye 568)

Catalog No. ABIN4368331

The Alpaca Monoclonal anti-RFP antibody has been validated for IF, FACS and ICC. It is suitable to detect RFP in samples from Discosoma.

Quick Overview for Recombinant RFP antibody (AZDye 568) (ABIN4368331)

Target: RFP (Red Fluorescent Protein (RFP))

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivity: Discosoma

Host: Alpaca

Clonality: Monoclonal

Conjugate: This RFP antibody is conjugated to AZDye 568

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 2B12

Product Details anti-RFP Antibody (AZDye 568)

Expression System: E.coli

Purpose: Camelid sdAb anti-RFP conjugated with AZDye 568 (Alexa Fluor 568 equivalent), Clone 2B12

Specificity: Recognizes most common red fluorescent proteins like mRFP and derivatives like mCherry, mScarlet-i, tdTomato, dsRed and mOrange.

Cross-Reactivity (Details): Does not cross-react with GFP or mTagBFP derivatives.

Characteristics: A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.

Purification: Produced in: E.coli

Immunogen: Recombinant protein corresponding to AA 1 to 225 from sea anemone RFP (UniProt Id: Q9U6Y8)

Labeling Ratio: a single fluorophore is coupled to exactly one sdAb

Application Details

Application Notes: Recommended dilution 1:500

Comment:

Each fluorophore is coupled to exactly one sdAb, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Concentration: 5 μM

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Handling Advice: Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.

Storage: -20 °C,-80 °C

Storage Comment: Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below

Expiry Date: 12 months

Target Details for RFP

Target: RFP (Red Fluorescent Protein (RFP))

Alternative Name: RFP

Molecular Weight: 26 kDa