Cytokeratin 1 antibody (pan)

Catalog No. ABIN452372

The Mouse Monoclonal anti-Cytokeratin 1 antibody has been validated for IHC (p), IF and IHC (fro). It is suitable to detect Cytokeratin 1 in samples from Human, Rat, Cow, Chicken, Snake, Xenopus laevis and Pleurodeles.

Quick Overview for Cytokeratin 1 antibody (pan) (ABIN452372)

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Reactivity: Human, Rat, Cow, Chicken, Snake, Xenopus laevis, Pleurodeles

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: Lu-5

Product Details anti-Cytokeratin 1 Antibody

Binding Specificity: pan

Specificity: This antibody reacts to Cytoketatin pan.

Characteristics: Synonyms: pan Keratin, Cytokeratin Pan-reactive

Purification: Ion exchange chromatography

Immunogen: Cells of lung cancer cell line A549 and A2182.

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen and paraffin sections: 1/20.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 1 mL dist. water

Buffer: 0.09 % sodium azide, 0.5 % BSA in PBS buffer, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Cytokeratin 1

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Alternative Name: Cytokeratin

Background: Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.Synonyms: Cytokeratin pan-reactive, pan Keratin