PPP2R4 antibody (C-Term)

Catalog No. ABIN452430

This anti-PPP2R4 antibody is a Goat Polyclonal antibody detecting PPP2R4 in WB and EIA. Suitable for Human, Mouse, Rat, Cow and Dog.

Quick Overview for PPP2R4 antibody (C-Term) (ABIN452430)

Target: PPP2R4 (Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This PPP2R4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PPP2R4 Antibody

Binding Specificity: C-Term

Sequence: c-GSLLPIHPVT SG

Specificity: This antibody is expected to recognize all reported isoforms (NP_821068.1, NP_066954.2, NP_821070.1).

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog), Bovine.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography.

Immunogen: Peptide with sequence from the C-Terminus of the protein sequence according to NP_821068.1, NP_066954.2, NP_821070.1. Genename: PPP2R4

Application Details

Application Notes: Peptide ELISA: 1/32000. Western Blot: Use at 0.5-2.0 μg/mL. Approx 37 kDa band observed in Human Liver lysates and in lysates of cell line HepG2(Calculated MW of 36.7 kDa according to NP_066954.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris-saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PPP2R4

Target: PPP2R4 (Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4))

Alternative Name: PPP2R4 / PTPA

Background: Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms.Synonyms: PP2A subunit B' PR53 isoform, Phosphotyrosyl phosphatase activator, Serine/threonine-protein phosphatase 2A regulatory subunit B'

Gene ID: 5524

NCBI Accession: NP_001180326

UniProt: Q15257

Pathways: PI3K-Akt Signaling, M Phase, Hepatitis C, Toll-Like Receptors Cascades