HCV NS4 antibody

Catalog No. ABIN452527

Product Details anti-HCV NS4 Antibody

Target: HCV NS4 (Hepatitis C Virus NS4 (HCV NS4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HCV NS4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: Hepatitis C Virus, Non-structural protein NS4A, NS4B

Purification: Affinity Chromatography on Protein G.

Immunogen: Synthetic polypeptide in which overlapping sequence of at least 90 residues in lengthwere selected from immunodominant NS-4 region of Chinese HCV strains.

Clone: 7B7-5G9

Isotype: IgG1

Application Details

Application Notes: ELISA: React with Human Hepatitis C Virus. Western Blot: Use of HCV NS4 antibody (clone 7B7/5G9) at 0.5 μg/mL will allowvisualization of 0.5-10 μg/lane of synthetic NS-4 peptide and 0.1 μg/lane of RecombinantChimeric HCV polyprotein. Testing is under both reducing and non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL.

Buffer: 0.01 M PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for HCV NS4

Target: HCV NS4 (Hepatitis C Virus NS4 (HCV NS4))

Alternative Name: HCV NS4 (HCV NS4 Products)

Target Type: Viral Protein

Background: NS4 A/B are two of the seven nonstructural (NS) proteins making up the HCV polyprotein. The NS proteins recruit the viral genome into an RNA replication complex, which is associated with rearranged cytoplasmic membranes. NS4A acts as a cofactor with the NS3 serine protease and stabilizes its folding. The NS3-NS4A complex is essential for the activation of the latter and allows membrane anchorage of NS3. HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. HCV is responsible for a large proportion of worldwide chronic viral hepatitides. Most of these infections develop into chronic hepatitis, which often progresses to liver cirrhosis and hepatocellular carcinoma. At present, (unlike hepatitis A and B), there is no vaccine to prevent hepatitis C infection. The hepatitis C virus (HCV) nonstructural protein 4B (NS4B) is a relatively hydrophobic 27- kDa protein. The 4A protein has a molecular weight of 6 kDa.Synonyms: Hepatitis C Virus, NS4B, Non-structural protein NS4A