ENO2/NSE antibody

Catalog No. ABIN452594

The Mouse Monoclonal anti-ENO2/NSE antibody has been validated for WB and EIA. It is suitable to detect ENO2/NSE in samples from Human.

Quick Overview for ENO2/NSE antibody (ABIN452594)

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ENO2/NSE antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 145

Product Details anti-ENO2/NSE Antibody

Specificity: Reacts with recombinant Human Neuron Specific Enolase (NSE)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: NSE, ENO2, Enolase 2, Neural enolase, Gamma-enolase, 2-phospho-D-glyceratehydro-lyase

Purification: Affinity Chromatography on Protein G.

Immunogen: Recombinant Human Neuron Specific Enolase (NSE)

Isotype: IgG2b

Application Details

Application Notes: ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL.

Buffer: 0.01 M PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for ENO2/NSE

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Alternative Name: Neuron Specific Enolase

Background: Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma in certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.Synonyms: 2-phospho-D-glycerate hydro-lyase, ENO2, Enolase 2, Gamma-enolase, NSE, Neural enolase

Gene ID: 2026

UniProt: P09104