CREB1 antibody (pSer133)

Catalog No. ABIN452620

This Mouse Monoclonal antibody specifically detects CREB1 in EIA. It exhibits reactivity toward Human.

Quick Overview for CREB1 antibody (pSer133) (ABIN452620)

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CREB1 antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Clone: B1C4

Product Details anti-CREB1 Antibody

Binding Specificity: pSer133

Specificity: Reactive to serine133 phosphorylated Human CREB peptide fragment, and reactive to KLH or BSA conjugated Serine133 phosphorylated Human CREB peptide fragment. Not reactive to un-conjugated KLH or BSA.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsiveelement-binding protein 1

Purification: Affinity Chromatography on Protein G.

Immunogen: Phosphorylated peptide fragment of CREB (pSer133), conjugated with KLH

Isotype: IgG2b

Application Details

Application Notes: Indirect ELISA: The suggested antigen coating quantity is 1 μg/mL of peptide/or peptideconjugating in pH 9.5 Carbonate buffer, 100 μL/well.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL.

Buffer: 0.01 M PBS, pH 7.0 without preservatives.

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. After reconstitution, aliquot and store at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CREB1

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Alternative Name: CREB1

Background: Transcription factor CREB binds the cAMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth or neurotrophic factors. Protein kinase A and the calmodulin-dependent protein kinase CaMKII stimulate CREB phosphorylation at Ser133, a key regulatory site controlling transcriptional activity. Phosphorylation at Ser133 is also mediated by p44/42 MAP kinase, p90RSK, p38 MAP kinase and MSK1. CREB appears to play an important role in learning and memory in both flies and mice.Synonyms: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1

Gene ID: 1385

UniProt: P16220

Pathways: TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation