SDHAF1 antibody (C-Term)

Catalog No. ABIN452676

The Goat Polyclonal anti-SDHAF1 antibody (ABIN452676) specifically detects SDHAF1 in WB and EIA. The antibody is reactive with Human, Mouse and Dog samples.

Quick Overview for SDHAF1 antibody (C-Term) (ABIN452676)

Target: SDHAF1 (Succinate Dehydrogenase Complex Assembly Factor 1 (SDHAF1))

Reactivity: Human, Mouse, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This SDHAF1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-SDHAF1 Antibody

Binding Specificity: C-Term

Sequence: c-HDSTGAPETR PDGR

Specificity: This antibody detects Human SDHAF1 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C-Terminus of the protein sequence according to NP_001036096.1. Genename: RNF31

Application Details

Application Notes: Peptide ELISA: Detection limit dilution 1/16000. Western blot: 0.3-1.0 μg/mL. Approx 19 kDa band observed in Human Brain (Cerebellum)lysates (calculated MW of 12.9 kDa according to NP_001036096.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for SDHAF1

Target: SDHAF1 (Succinate Dehydrogenase Complex Assembly Factor 1 (SDHAF1))

Alternative Name: SDHAF1

Background: The succinate dehydrogenase (SDH) complex (or complex II) of the mitochondrial respiratory chain is composed of 4 individual subunits. The protein encoded by this gene resides in the mitochondria, and is essential for SDH assembly, but does not physically associate with the complex in vivo. Mutations in this gene are associated with SDH-defective infantile leukoencephalopathy (mitochondrial complex II deficiency). SDHAF1 Plays an essential role in succinate dehydrogenase complex (SDH) assembly, a complex involved in complex II of the mitochondrial electron transport chain. Probably acts by participating in mitochondrial biosynthesis of iron-sulfur centers for complex II (Probable)Synonyms: LYR motif-containing protein 8, SDH assembly factor 1, Succinate dehydrogenase assembly factor 1 mitochondrial

Gene ID: 644096

NCBI Accession: NP_001036096

UniProt: A6NFY7