FOXE1 antibody (C-Term)

Catalog No. ABIN452689

Product Details anti-FOXE1 Antibody

Target: FOXE1 (Forkhead Box E1 (Thyroid Transcription Factor 2) (FOXE1))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FOXE1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: Recognizes FOXE1 / TTF2 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Bat, Elephant, Marmoset, Gorilla, Monkey, Rabbit.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-AYPGGIDRFVSAM, from the C Terminus of the protein sequence according to NP_004464.2. Genename: FOXE1

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/32000. Western blot: Preliminary experiments gave an approx 70 kDa band in Human Thymus andThyroid Gland lysates after 0.3 μg/mL antibody staining. Please note that currently wecannot find an explanation in the literature for the band we observe given the calculated

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for FOXE1

Target: FOXE1 (Forkhead Box E1 (Thyroid Transcription Factor 2) (FOXE1))

Alternative Name: FOXE1 / FKHL15 (FOXE1 Products)

Synonyms: FKHL15 antibody, FOXE2 antibody, HFKH4 antibody, HFKL5 antibody, TITF2 antibody, TTF-2 antibody, TTF2 antibody, Titf2 antibody, Ttf-2 antibody, Ci-FoxE antibody, Foxe1 antibody, FoxE5 antibody, ttf2 antibody, foxe2 antibody, hfkh4 antibody, hfkl5 antibody, titf2 antibody, ttf-2 antibody, fkhl15 antibody, foxE1 antibody, foxE antibody, forkhead box E1 antibody, foxE protein antibody, forkhead box E1 L homeolog antibody, FOXE1 antibody, Foxe1 antibody, foxE antibody, foxe1.L antibody, foxe1 antibody, foxE1 antibody

Background: Forkhead box protein E1 (FOXE1) is a member of the forkhead/ winged-helix domain transcription factor family. FOXE1, also designated FKHL15 or TTF-2, complexes with TTF-1 and Pax-8 to induce thyroid follicular cell differentiation and thyroid hormone biosynthesis by regulating the expression of the sodium iodide symporter (NIS), thyroid peroxidase (TPO), thyroglobulin (TG) and the thyrotropin receptor (TSHR). FOXE1 encodes a protein that is expressed in several tissues, including thymus, adult brain, lung, liver, heart and pancreas. The chromosomal location of the FOXE1 gene on 9q22 suggests that it may be involved in squamous cell epithelioma and hereditary sensory neuropathy type I. Mutations in the FOXE1 gene lead to the development of congenital hypothyroidism, which occurs in approximately one in four thousand newborns and results in complete or partial failure of thyroid gland development. Patients who are homozygous for a missense mutation in the forkhead domain of he FOXE1 gene can also develop thyroid agenesis, cleft palate and choanal atresia. Subsequently, the FOXE1 gene may used as a marker to study these disorders.Synonyms: FOXE2, Forkhead box protein E1, HFKH4, HFKL5, HNF-3/fork head-like protein 5, TITF2, TTF-2, TTF2, Thyroid transcription factor 2

Gene ID: 2304

NCBI Accession: NP_004464

UniProt: O00358