APRT antibody (N-Term)

Catalog No. ABIN452753

The Rabbit Polyclonal anti-APRT antibody is suitable to detect APRT in samples from Human. It has been validated for WB, IHC (p) and EIA.

Quick Overview for APRT antibody (N-Term) (ABIN452753)

Target: APRT (Adenine Phosphoribosyltransferase (APRT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APRT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-APRT Antibody

Binding Specificity: N-Term

Specificity: This antibody detects APRT at N-term.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Purified through a protein A column, followed by peptide affinity purification

Immunogen: KLH conjugated synthetic peptide selected from the N-terminal region of human APRT

Application Details

Application Notes: Western blot: 1: 100 - 1: 500. ELISA: 1: 1,000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for APRT

Target: APRT (Adenine Phosphoribosyltransferase (APRT))

Alternative Name: APRT

Background: A conserved feature of APRT is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis.Synonyms: Adenine phosphoribosyltransferase

Molecular Weight: 19608 Da

Gene ID: 353

NCBI Accession: NP_000476

UniProt: P07741

Pathways: Ribonucleoside Biosynthetic Process