PLEKHO1 antibody (N-Term)

Catalog No. ABIN452845

This Rabbit Polyclonal antibody specifically detects PLEKHO1 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for PLEKHO1 antibody (N-Term) (ABIN452845)

Target: PLEKHO1 (Pleckstrin Homology Domain Containing, Family O Member 1 (PLEKHO1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLEKHO1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PLEKHO1 Antibody

Binding Specificity: N-Term

Specificity: This antibody detects CKIP-1 at N-term.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS

Immunogen: KLH conjugated synthetic peptide selected from the N-terminal region of human CKIP-1

Application Details

Application Notes: Western blot: 1: 50 - 1: 100. ELISA: 1: 1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for PLEKHO1

Target: PLEKHO1 (Pleckstrin Homology Domain Containing, Family O Member 1 (PLEKHO1))

Alternative Name: PLEKHO1 / CKIP1

Background: CKIP-1 plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). This protein may function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). It appears to target ATM to the plasma membrane and appears to also inhibit tumor cell growth by inhibiting AKT-mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation.Synonyms: C-Jun-binding protein, CK2-interacting protein 1, CKIP-1, Casein kinase 2-interacting protein 1, HQ0024c, JBP, OC120, Osteoclast maturation-associated gene 120 protein, PH domain-containing family O member 1, Pleckstrin homology domain-containing family O member 1

Molecular Weight: 46237 Da

Gene ID: 51177

NCBI Accession: NP_057358

UniProt: Q53GL0