eEF1A1 antibody (C-Term)
Quick Overview for eEF1A1 antibody (C-Term) (ABIN452935)
Target
See all eEF1A1 (EEF1A1) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- C-Term
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Specificity
- This antibody detects Elongation factor 1 A-1 at C-term.
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Cross-Reactivity (Details)
- Species reactivity (tested):Human
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Purification
- Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
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Immunogen
- KLH conjugated synthetic peptide selected from the C-terminal region of human EEF1A1
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Application Notes
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Western blot: 1: 50 - 1: 100. ELISA: 1: 1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.25 mg/mL
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Buffer
- PBS with 0.09 % (W/V) sodium azi
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid repeated freezing and thawing.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
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- eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))
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Alternative Name
- EEF1A1 / LENG7
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Background
- EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66 % of patients with Felty syndrome.Synonyms: CCS-3, CCS3, EEF1A, EF-1-alpha-1, EF-Tu, EF1A, Elongation factor 1 alpha 1, Elongation factor Tu, Eukaryotic elongation factor 1 A-1, FLJ25721, GRAF-1EF, Leukocyte receptor cluster member 7, PTI1, eEF1A-1
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Molecular Weight
- 50141 Da
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Gene ID
- 1915
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NCBI Accession
- NP_001393
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UniProt
- P68104
Target
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