SRSF2 antibody (Middle Region)

Catalog No. ABIN453825

The Rabbit Polyclonal anti-SRSF2 antibody (ABIN453825) specifically detects SRSF2 in WB and EIA. The antibody is reactive with Human samples.

Quick Overview for SRSF2 antibody (Middle Region) (ABIN453825)

Target: SRSF2 (serine/arginine-Rich Splicing Factor 2 (SRSF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SRSF2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-SRSF2 Antibody

Binding Specificity: AA 83-113, Middle Region

Specificity: This antibody reacts to SFRS2.

Purification: Saturated Ammonium Sulfate (SAS) precipitation

Immunogen: KLH conjugated synthetic peptide selected from aa 83-113 from the Center region of human SFRS2

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SRSF2

Target: SRSF2 (serine/arginine-Rich Splicing Factor 2 (SRSF2))

Alternative Name: SFRS2 / SC-35

Background: SFRS2 is necessary for the splicing of pre-mRNA. The protein is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. And it interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3' and can bind to beta-globin mRNA and commit it to the splicing pathway.Synonyms: Nuclear Speckle Marker, PR264, Splicing component 35 kDa, Splicing factor SC35, Splicing factor arginine/serine-rich 2

Gene ID: 6427

NCBI Accession: NP_003007

UniProt: Q01130