CCL2 antibody (HRP)

Catalog No. ABIN453918

The HRP-conjugated Mouse Monoclonal anti-CCL2 antibody (Clone S101) (ABIN453918) specifically detects CCL2 in EIA. The antibody is reactive with Human samples.

Quick Overview for CCL2 antibody (HRP) (ABIN453918)

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL2 antibody is conjugated to HRP

Application: Enzyme Immunoassay (EIA)

Clone: S101

Product Details anti-CCL2 Antibody (HRP)

Specificity: This monoclonal antibody reacts with natural and recombinant Human MCP-1. Does not react with Human interleukin-8 (IL-8) and other human cytokines tested such as interleukin-1 beta (IL1 beta), serum amyloid A (SAA) and epidermal growth factor (EGF)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G

Immunogen: Purified recombinant Human MCP1.

Isotype: IgG1

Application Details

Application Notes: ELISA: This MCP1 antibody HRP-conjugated can be used as a Tracer/Detection antibody inSandwich ELISA applications for Human MCP-1 detection in combination with a Captureantibody (Cat#DM2032)Suggested Capture Coating Dose: approx. 0.3 μg/mLSubstrate: TMB. If the above suggested conditions are followed approximately 1.5 pg/mL ofMCP-1 in serum/plasma or 4 pg/mL of MCP-1 in medium can be detected with an assayrange of 0-1600 pg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01 M PBS, pH 7.0 ± 0.1 in 50 % Glycerol and 0.01 % Thimerosal as a bacteriostat

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for CCL2

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Alternative Name: MCP1 / CCL2

Background: Monocyte chemotactic protein-1 (MCP-1), also known as CCL2, monocyte chemotactic activating factor (MCAF) or glioma-derived chemotactic factor-2 (GDCF-2), is the product of the human JE gene and a member of the family of C-C (or beta) chemokines (1-4). The predicted molecular weight of MCP-1 protein is 11-13 kDa, but it may migrate at 20-30 kDa due to glycosylation. MCP-1 is secreted by a variety of cell types in response to pro-inflammatory stimuli and was originally described for its chemotactic activity on monocytes. This activity has led to studies demonstrating its role in diseases characterized by monocyte infiltrates such as psoriasis (5), rheumatoid arthritis (6) and atherosclerosis (7). MCP-1 may also contribute to tumor progression and angiogenesis (8). Signaling by MCP-1 is mediated by the G-protein coupled receptor CCR2 (9).Synonyms: C-C motif chemokine 2, HC-11, HC11, MCAF, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small-inducible cytokine A2

Gene ID: 6347

NCBI Accession: NP_002973

UniProt: P13500

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection