HMOX1 antibody (N-Term)

Catalog No. ABIN453936

The Mouse Monoclonal anti-HMOX1 antibody (Clone 1F12-A6) (ABIN453936) specifically detects HMOX1 in WB, IP, IF, IHC (fro) and EIA. The antibody is reactive with Human, Mouse and Cow samples.

Quick Overview for HMOX1 antibody (N-Term) (ABIN453936)

Target: HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))

Reactivity: Human, Mouse, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This HMOX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 1F12-A6

Product Details anti-HMOX1 Antibody

Binding Specificity: AA 1-30, N-Term

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Bovine.

Characteristics: Synonyms: HO-1, HO, HO1

Purification: Protein G purified

Immunogen: Human heme-oxygenase (HO-1) synthetic peptide, amino acids 1-30

Isotype: IgG1

Application Details

Application Notes: Western blot: 1: 500 - 1000. Immunohistochemistry on frozen sections. Immunoprecipitation.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS pH 7.4, 0.09 % Sodium Azide, 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for HMOX1

Target: HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))

Alternative Name: Heme Oxygenase 1 / HMOX1

Background: Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator, biliverdin and bilirubin are potent antioxidants, and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3, however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Upregulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).Synonyms: HO, HO-1, HO1

Gene ID: 3162

UniProt: P09601

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, SARS-CoV-2 Protein Interactome