CRYAA antibody

Catalog No. ABIN453938

The Mouse Monoclonal anti-CRYAA antibody is suitable to detect CRYAA in samples from Human, Mouse, Rat and Cow. It has been validated for WB and EIA.

Quick Overview for CRYAA antibody (ABIN453938)

Target: CRYAA (Crystallin, alpha A (CRYAA))

Reactivity: Human, Mouse, Rat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This CRYAA antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 1H3-B8

Product Details anti-CRYAA Antibody

Specificity: This antibody recognizes Alpha A Crystallin at ~20 kDa. Detects alpha Crystallin complex (containing Alpha A and Alpha B subunits). Does not cross react with Alpha B Crystallin, Beta-L Crystallin, Beta-H Crystallin, Gamma Crystallin, Hsp25, Hsp27 or Hsp47 proteins.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Cow, Rat.

Characteristics: Synonyms: CRYAA, CRYA1, HSPB4, Heat shock protein beta-4

Purification: Protein G affinity chroamtography

Immunogen: Native alpha crystallin

Isotype: IgG1

Application Details

Application Notes: Western blot: 1/2000. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS pH 7.2, 0.09 % Sodium Azide, 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CRYAA

Target: CRYAA (Crystallin, alpha A (CRYAA))

Alternative Name: alpha-Crystallin A Chain

Background: The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alphacrystallins possess structural and functional similarities with Hsp25 and Hsp27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8).Synonyms: CRYA1, CRYAA, HSPB4, Heat shock protein beta-4

Gene ID: 1409

UniProt: P02489

Pathways: M Phase