Hemoglobin antibody

Catalog No. ABIN453960

The Sheep Polyclonal anti-Hemoglobin antibody (ABIN453960) specifically detects Hemoglobin in WB and EIA. The antibody is reactive with Human and Cow samples.

Quick Overview for Hemoglobin antibody (ABIN453960)

Target: Hemoglobin

Reactivity: Human, Cow

Host: Sheep

Clonality: Polyclonal

Conjugate: This Hemoglobin antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Hemoglobin Antibody

Cross-Reactivity (Details): Species reactivity (tested):Human and Bovine (weak).

Purification: Affinity Chromatography on Protein G.

Immunogen: Native Hemoglobin from Erythrocytes.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: 1/100-1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 5.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Hemoglobin

Target: Hemoglobin

Background: Hemoglobin is a 66 kDa metalloprotein composed of four globular subunits each of which is associated with an iron-containing heme group. The heme group is involved with oxygen transport from the lungs to the tissues and the globin molecule plays a major role in transporting carbon dioxide in the reverse direction. Hemoglobin is found in the erythrocytes of all vertebrates. There is a wide diversity of amino acid sequences and substitutions within mammalian hemoglobins, however the molecular weight is generally around 66 kDa with an iron content of about 0.34 % . The level of hemoglobin in the blood is used in the diagnosis of anemia, levels in the feces are indicative of various clinical conditions. Mutations in the genes for the hemoglobin protein result in a group of hereditary diseases in humans termed hemoglobinopathies, and include sickle-cell disease and thalassemias.Synonyms: Haemoglobin