CYB5R3 antibody (C-Term)

Catalog No. ABIN453978

Cited in 3+ publications. The Goat Polyclonal anti-CYB5R3 antibody (ABIN453978) specifically detects CYB5R3 in WB and EIA. The antibody is reactive with Rat, Mouse, Dog and Cow samples.

Quick Overview for CYB5R3 antibody (C-Term) (ABIN453978)

Target: CYB5R3 (Cytochrome B5 Reductase 3 (CYB5R3))

Reactivity: Rat, Mouse, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This CYB5R3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CYB5R3 Antibody

Binding Specificity: C-Term

Specificity: This antibody recognizes CYB5R3.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Canine (Dog) and Cow.
Species reactivity (tested):Mouse.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence PNLERVGHPKERC, from the C-Terminus of the protein sequence according to NP_084063.1.

Application Details

Application Notes: Peptide ELISA: Detection limit dilution 1/32000. Western blot: 0.1-1 μg/mL. Approx 35 kDa band observed in Mouse Liver lysates as well as inMouse and Rat brain lysates (calculated MW of 34.5 kDa according to Mouse NP_084063.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Species Reactivity: Tested: Mouse. Expected from sequence similarity: Rat, Canine (Dog) and Cow.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-CYB5R3 antibody (ABIN453978)

Reed, Jarvis, Phillips, Arlt: "Enhanced DNA adduct formation by benzo[a]pyrene in human liver cells lacking cytochrome P450 oxidoreductase." in: Mutation research, Vol. 852, pp. 503162, (2020) (PubMed).

Reed, Indra, Mrizova, Moserova, Schmeiser, Wolf, Henderson, Stiborova, Phillips, Arlt: "Application of hepatic cytochrome b5/P450 reductase null (HBRN) mice to study the role of cytochrome b5 in the cytochrome P450-mediated bioactivation of the anticancer drug ellipticine." in: Toxicology and applied pharmacology, Vol. 366, pp. 64-74, (2019) (PubMed).

Reed, Mrizova, Barta, Indra, Moserova, Kopka, Schmeiser, Wolf, Henderson, Stiborova, Phillips, Arlt: "Cytochrome b 5 impacts on cytochrome P450-mediated metabolism of benzo[a]pyrene and its DNA adduct formation: studies in hepatic cytochrome b 5 /P450 reductase null (HBRN) mice." in: Archives of toxicology, Vol. 92, Issue 4, pp. 1625-1638, (2019) (PubMed).

Target Details for CYB5R3

Target: CYB5R3 (Cytochrome B5 Reductase 3 (CYB5R3))

Alternative Name: CYB5R3

Background: CYB5R3 belongs to the flavoprotein pyridine nucleotide cytochrome reductase family and contains 1 FAD-binding FR-type domain. There are two isoforms, NADH-cytochrome b5 reductase 3 membrane bound form and NADH-cytochrome b5 reductase 3 soluble form. Its functions include desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Defects in CYB5R3 are the cause of hereditary methemoglobinemia (HM). There are three forms of this disease: type 1 (HM1) in which the enzyme is only deficient in erythrocytes with a mild cyanosis, type 2 (HM2), in which the enzyme is completely deficient, type 3 (HM3) where the deficiency is seen in all blood cells. Type 2 is a severe form accompanied with mental retardation and neurological impairment.Synonyms: B5R, DIA1, Diaphorase-1, NADH-cytochrome b5 reductase 3

Gene ID: 1727

NCBI Accession: NP_000389

UniProt: P00387

Pathways: SARS-CoV-2 Protein Interactome