ENPP3 antibody (PE)

Catalog No. ABIN457373

There are 4+ publications for this product available. The Mouse Monoclonal anti-ENPP3 antibody is suitable to detect ENPP3 in samples from Human. It has been validated for FACS and ICC.

Quick Overview for ENPP3 antibody (PE) (ABIN457373)

Target: ENPP3 (Ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ENPP3 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: NP4D6

Product Details anti-ENPP3 Antibody (PE)

Purpose: Anti-Hu CD203c PE

Cross-Reactivity (Details): Human

Characteristics: The mouse monoclonal antibody NP4D6 reacts with an extracellular epitope of CD203c, a transmembrane ectoenzyme expressed on basophils and mast cells, and overexpressed upon their activation.

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: HEK-293 cells transfected with human CD203c

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. Extracellular and intracellular staining.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze,Protect from prolonged exposure to light

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-ENPP3 antibody (PE) (ABIN457373)

Heneberg, Riegerová, Kučera: "Pimecrolimus Is a Potent Inhibitor of Allergic Reactions to Hymenopteran Venom Extracts and Birch Pollen Allergen In Vitro." in: PLoS ONE, Vol. 10, Issue 11, pp. e0142953, (2015) (PubMed).

Platz, Binder, Marxer, Lischka, Valent, Bühring: "Hymenoptera-venom-induced upregulation of the basophil activation marker ecto-nucleotide pyrophosphatase/phosphodiesterase 3 in sensitized individuals." in: International archives of allergy and immunology, Vol. 126, Issue 4, pp. 335-42, (2002) (PubMed).

Bühring, Seiffert, Giesert, Marxer, Kanz, Valent, Sano: "The basophil activation marker defined by antibody 97A6 is identical to the ectonucleotide pyrophosphatase/phosphodiesterase 3." in: Blood, Vol. 97, Issue 10, pp. 3303-5, (2001) (PubMed).

Bühring, Simmons, Pudney, Müller, Jarrossay, van Agthoven, Willheim, Brugger, Valent, Kanz: "The monoclonal antibody 97A6 defines a novel surface antigen expressed on human basophils and their multipotent and unipotent progenitors." in: Blood, Vol. 94, Issue 7, pp. 2343-56, (1999) (PubMed).

Target Details for ENPP3

Target: ENPP3 (Ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3))

Alternative Name: CD203c

Background: Ectonucleotide pyrophosphatase/phosphodiesterase 3,CD203c, also known as ENPP-3, is integral membrane ectoenzyme (ectonucleotide pyrophosphatase/phosphodiesterase 3), that hydrolyses nucleotide triphosphates and thus modulates purinergic signaling. CD203c is expressed mainly on activated basophils and mast cells. CD203c is upregulated in response to IgE-receptor cross-linking and is overexpressed on neoplastic mast cells in patients with systemic mastocytosis. Measurement of its induced enhancement on the plasma membrane is useful for diagnostics of allergies.,ENPP3, B10, PDNP3, PD-IBETA

Gene ID: 5169

UniProt: O14638

Pathways: Regulation of Muscle Cell Differentiation, Negative Regulation of Transporter Activity