kappa Light Chain antibody

Catalog No. ABIN457414

This Mouse Monoclonal antibody specifically detects kappa Light Chain in FACS, IHC (p), ELISA and IHC (fro). It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for kappa Light Chain antibody (ABIN457414)

Target: kappa Light Chain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This kappa Light Chain antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: A8B5

Product Details anti-kappa Light Chain Antibody

Purpose: Anti-Hu Ig Kappa Light Chain Purified

Specificity: The mouse monoclonal antibody A8B5 reacts with kappa light chains (22.5 kDa) of immunoglobulins.

No Cross-Reactivity: Goat, Guinea Pig, Hamster, Rabbit, Sheep

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL, Positive control: Daudi human cell line.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-kappa Light Chain antibody (ABIN457414)

Scheeren, Diehl, Smit, Beaumont, Naspetti, Bende, Blom, Karube, Ohshima, van Noesel, Spits: "IL-21 is expressed in Hodgkin lymphoma and activates STAT5: evidence that activated STAT5 is required for Hodgkin lymphomagenesis." in: Blood, Vol. 111, Issue 9, pp. 4706-15, (2008) (PubMed).

Konig, Wennemuth, Soyer, Hoffmann, Happle, Krause: "Vulvar amyloidosis mimicking giant condylomata acuminata in a patient with multiple myeloma." in: European journal of dermatology : EJD, Vol. 9, Issue 1, pp. 29-31, (1999) (PubMed).

Target Details for kappa Light Chain

Target: kappa Light Chain

Alternative Name: Ig Kappa Light Chain

Background: Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.,Immunoglobulin kappa, Igk