kappa Light Chain antibody

Details for Product No. ABIN94406
Antigen
Reactivity
Human
79
6
Host
Mouse
69
12
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
10
9
6
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
53
32
19
14
14
11
5
4
3
3
2
2
1
1
Options
Immunogen Crude thymus membrane fraction.
Clone MEM-09
Isotype IgG1
Specificity The antibody MEM-09 reacts with both secreted and B cell-surface human immunoglobulin, specifically reacts with kappa light chains (22.5 kDa).
Material immunoprecipitated from human serum with the antibody MEM-09 consists of IgG and traces of IgM.
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name Kappa light chains
Background Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-kappa Light Chain antibody (ABIN94406) Western Blotting analysis (reducing conditions) of human blood sera using anti-human ...