Claudin 1 antibody (C-Term)

Catalog No. ABIN457476

The Rabbit Polyclonal anti-Claudin 1 antibody (ABIN457476) specifically detects Claudin 1 in WB, IHC, ELISA and IHC (p). The antibody is reactive with Human, Mouse, Rat, Dog and Monkey samples.

Quick Overview for Claudin 1 antibody (C-Term) (ABIN457476)

Target: Claudin 1 (CLDN1)

Reactivity: Human, Mouse, Rat, Dog, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Claudin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Claudin 1 Antibody

Binding Specificity: C-Term

Specificity: Recognizes Claudin 1. Species cross-reactivity: Human, Monkey, Mouse, Canine, and Rat.

Purification: Immunoaffinity purified

Immunogen: A synthetic peptide derived from the C-terminus of human Claudin 1.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 2-4 μg/mL for 2 hrs at RT. Positive control: T47D cells, Skin or Breast carcinoma.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM PBS,  pH 7.6, 0.2 % BSA, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Claudin 1

Target: Claudin 1 (CLDN1)

Alternative Name: CLDN1 / Claudin 1

Background: Name/Gene ID: CLDN1
Family: Claudin

Synonyms: CLDN1, Claudin-1, Claudin 1, ILVASC, SEMP1, CLD1

Gene ID: 9076

UniProt: O95832

Pathways: Cell-Cell Junction Organization, Hepatitis C