Beta Lactoglobulin (LGB) antibody

Catalog No. ABIN457578

Product Details

Target: Beta Lactoglobulin (LGB)

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID), Nephelometry (Neph), Immunodiffusion (ID)

Specificity: Homologous proteins of different species frequently share antigenic determinants. has been observed with milk of sheep and goat.

Characteristics: Precipitating polyclonal rabbit antiserum to bovine beta-lactoglobulin

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Beta-lactoglobulin is the dominant protein in bovine milk, synthesized in mammary gland tissue. It is a glycoprotein present mainly as a dimer at intermediate concentrations near the isoelectric point. The molecular weight of the monomeric subunit is near 17,000. It is very sensitive to denaturation. Any treatment of native milk and also prolonged storage in the deepfreeze will lead first to polymerization (octamers and higher polymers) aggregation and finally to denaturation. Three genetic variants A, B and C have been identified. They differ very little in the amino acid constellation an their behavior under pH and temperature various conditions. No immunological differences was found between the variants. Beta-lactoglobulin can be purified in crystallized form. Allergic reactions of infants to bovine milk proteins are well known. The actual process by which such reactions develop and their impact on the infant’s health have not been unambiguously established. Circulating antibodies to beta-lactoglobulin have been demonstrated in sera of some infants. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: As precipitating antiserum to identify or measure bovine beta-lactoglobulin by a variety of immunodiffusion techniques, including immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) and electroimmunodiffusion (Laurell). It has not been tested for use in nephelometry, ELISA or immunochemistry, but this does not exclude such use if proper controls are included.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those in pooled rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:16 when tested against normal bovine milk in agar block immunodiffusion titra

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Target Details

Target: Beta Lactoglobulin (LGB)

Alternative Name: beta-Lactoglobulin (LGB Products)

Synonyms: LGB antibody, GD antibody, GdA antibody, GdF antibody, GdS antibody, PAEG antibody, PEP antibody, PP14 antibody, Beta-LG antibody, progestagen-associated endometrial protein antibody, progestagen associated endometrial protein antibody, PAEP antibody

Background: The defined antibody specificity is directed to beta-lactoglobulin as tested against bovine milk. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) no reaction has been obtained with bovine serum