Ovalbumin antibody
Quick Overview for Ovalbumin antibody (ABIN457632)
Target
See all Ovalbumin (OVA) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Specificity
- Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.
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Characteristics
- Precipitating polyclonal rabbit antiserum to chicken ovalbumin
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Immunogen
- Highly purified egg albumin (MW 45,000) isolated from chicken egg whites. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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Isotype
- IgG
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Application Notes
- Immunoprecipitation. Precipitin titre not less than 1:16 when tested against an 1% solution of chicken egg albumin in agar-block immunodiffusion titration.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Concentration
- Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:16 when tested against an 1% solution of chicken egg albumin in agar-b
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Buffer
- Delipidated, heat inactivated, lyophilized, stable whole serum.
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Preservative
- Without preservative
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Storage
- 4 °C/-20 °C
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Storage Comment
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.
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- Ovalbumin (OVA)
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Alternative Name
- Ovalbumin
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Background
- A single precipitin line is obtained in immunoelectrophoresis against 1% chicken egg albumin in PBS. In double radial immunodiffusion of the antiserum against twofold dilutions of 1% chicken egg albumin in PBS and in the reverse arrangement of the chicken egg albumin solution against twofold dilutions of the antiserum, a singe precipitin line is obtained. The antiserum does not react with chicken serum
Target
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