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Mouse anti-Human IgG1 (Fc Region) Antibody (HRP)

ELISA, WB Monoclonal NI 132 (HP 6186) IgG1 kappa HRP

Catalog No. ABIN457836

Datasheet as PDF

Target See all IgG1 products

IgG1
Binding Specificity
41

25

9

6

4

3

3

2

2

1

1

1

1

1
Fc Region
Reactivity
95

24

23

9

7

5

4

3

3

2

1
Human
Host
60

42

39

15

10

6

1
Mouse
Clonality
92

60
Monoclonal
Conjugate
46

21

20

18

11

8

7

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
HRP
Application
89

59

51

45

30

19

18

16

14

11

10

9

8

6

4

3

2

2

2
ELISA, Western Blotting (WB)

Specificity

The reactivity of the antiserum is restricted to the subclass specific determinant on the Fc portion of the IgG1 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunoperoxidase staining of cytoplasmic Ig. Peroxidase conjugated purified monoclonal mouse antibody to human IgG1, subclass specific

Characteristics

Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).

Purification

Purified

Immunogen

Highly purified monoclonal IgG1 isolated from pooled human serum.

Clone

NI 132 (HP 6186)

Isotype

IgG1 kappa
Mouse anti-Human IgG1 (Hinge Region) antibody
ELISA Monoclonal 4E3 IgG1 unconjugated

Mouse anti-Human IgG1 (Hinge Region) antibody (HRP)
ELISA Monoclonal 4E3 IgG1 HRP

Mouse anti-Human IgG1 (Hinge Region) antibody (FITC)
ELISA Monoclonal 4E3 IgG1 FITC

Mouse anti-Human IgG1 (Hinge Region) antibody (PE)
ELISA Monoclonal 4E3 IgG1 PE

Mouse anti-Human IgG1 (Fc Region) antibody
ELISA, FLISA Monoclonal HP6001 IgG2b unconjugated

Mouse anti-Human IgG1 (Hinge Region) antibody (Alkaline Phosphatase (AP))
ELISA Monoclonal 4E3 IgG1 Alkaline Phosphatase (AP)

Mouse anti-Human IgG1 (Fc Region) antibody (Alkaline Phosphatase (AP))
ELISA, FLISA Monoclonal HP6001 IgG2b Alkaline Phosphatase (AP)

Mouse anti-Human IgG1 (Hinge Region) antibody (Biotin)
ELISA Monoclonal 4E3 IgG1 Biotin

Mouse anti-Human IgG1 (Fc Region) antibody (HRP)
ELISA, FLISA Monoclonal HP6001 IgG2b HRP

Mouse anti-Human IgG1 (Fc Region) antibody (PE)
ELISA, FLISA Monoclonal HP6001 IgG2b PE

Application Notes

To identify the presence of IgG1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG1, and immunoblotting.
The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:40, in ELISA from 1:125 to 1:8,000, in Western blotting from 1:250 to 1:10.000. These data should be interpreted as general recommendations only.

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.

Concentration

0.4 mg/mL

Buffer

Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH 7.2).

Preservative

Without preservative

Handling Advice

Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage

4 °C/-20 °C

Storage Comment

The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
Target

IgG1

Abstract

IgG1 Products

Target Type

Antibody