Bence-Jones Protein lambda (Chain lambda), (Free & Bound) antibody (Biotin)

Catalog No. ABIN457853

The Mouse Monoclonal anti-Bence-Jones Protein lambda antibody is suitable to detect Bence-Jones Protein lambda in samples from Human. It has been validated for ELISA and WB.

Quick Overview for Bence-Jones Protein lambda (Chain lambda), (Free & Bound) antibody (Biotin) (ABIN457853)

Target: Bence-Jones Protein lambda

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: ELISA, Western Blotting (WB)

Clone: NI 412 and NI 268

Product Details

Binding Specificity: Chain lambda, Free & Bound

Specificity: The antibody does not react with any other component of the human immuno-globulin system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.

Characteristics: Biotin-conjugated purified monoclonal mouse antibody to human Ig lambda chain, free and bound

Purification: Purified

Immunogen: Highly purified Bence Jones lambda proteins isolated from pooled human urine.

Isotype: IgG1

Application Details

Application Notes: To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100, in ELISA from 1:100 upwards, in Western blotting from 1:200 upwards. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG concentration is 0.4 mg/ml. Biotin/IgG protein molar ratio (B/P) is approximately 2.7. No foreign proteins added.

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Target Details

Target: Bence-Jones Protein lambda

Alternative Name: Bence Jones lambda

Background: The reactivity of the this preparation of two monoclonal antibodies is restricted to polyclonal and monoclonal immunoglobulins of the lambda type, as well as free lambda light chains, as tested in direct binding enzyme immunoassay, immuno-blotting, immunoprecipitation and indirect immunoperoxidase staining.