Mouse anti-Human IgD Antibody (HRP)

Catalog No. ABIN457875

Quick Overview for Mouse anti-Human IgD Antibody (HRP) (ABIN457875)

Target: IgD

Clonality: Monoclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB)

Clone: NI 158

Isotype: IgG1 kappa

Product Details

Reactivity: Human

Host: Mouse

Characteristics: The reactivity of the this monoclonal antibody is restricted to polyclonal and monoclonal IgD, as tested in direct binding enzyme immunoassay, immuno-blotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic immunoglobulins. Horseradish peroxidase conjugated purified monoclonal mouse antibody to human IgD, isotype specific
Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).

Purification: Purified

Immunogen: Highly purified polyclonal IgD isolated from human serum.

Application Details

Application Notes: To identify the presence of IgD in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:500, in ELISA from 1:1000 upwards, in Western blotting from 1:2000 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.

Concentration: 0.4 mg/mL

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH 7.2).

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Target Details

Target: IgD

Target Type: Antibody