Mouse anti-Human IgD Antibody

Catalog No. ABIN457925

Product Details

Target: IgD

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Specificity: The reactivity of the antiserum is restricted to the IgD molecule as tested in ELISA, immunoblotting, immunoprecipitation. Precipitation takes place under special test conditions in the presence of polyethylene glycol. Monoclonal mouse antibody to human IgD, isotype specific

Purification: Delipidated, heat inactivated, lyophilized, stable whole ascites

Immunogen: Highly purified monoclonal IgD isolated from human serum

Clone: NI 158

Isotype: IgG1 kappa

Application Details

Application Notes: To identify the presence of IgD in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immuno-peroxidase and immunofluorescence staining of cytoplasmic IgD and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100, in ELISA from 1:25 upwards, in Western blotting from 1:50 upwards. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized ascites by adding 0. 5 mL sterile distilled water.

Concentration: 1 mg/mL

Buffer: No foreign proteins added.

Handling Advice: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgD

Abstract: IgD Products

Target Type: Antibody